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Leon's whole job in Resident Evil 4 was to find Ashley Graham, the sitting president's daughter, and get her back to safety. That's exactly what he did after beating Saddler, and while no other piece of media showed what happened to Leon directly after the events of the Las Plagas incident in Valdelobos, Spain, we know that Leon refused Ashley's intent to have him as part of her security detail, and he resumed his career as a member of the Anti-Umbrella Pursuit and Investigation Unit until he was recruited into the DSO (Division of Security Operations). Leon's next major appearance was in Resi

Unlike Leon, Ashley never made another major appearance after the events of the game. The next time fans get to see her in the in-universe's canonical timeline is during the Infinite Darkness movie, where a photo of Ashley was briefly shown during a scene set in President Graham's office. We can only assume that after Resident Evil 4, Ashley resumed her life as an ordinary person. She was kidnapped while she was still a university student, so it's safe to say that she may have continued her studies after being rescued by Leon. Throughout RE4, Ashley mentioned she was interested in becoming an

The battle against Salazar in the Castle ultimately led to the destruction of the Queen Plaga, which is the main source of the Plaga parasites. Despite this, Las Plagas still exists in the Resident Evil universe, albeit in a significantly different form. Surviving members of the Los Ilumindaos cults still carry Plaga parasites within them, and members of the cult eventually collaborated with bioweapons dealer Glenn Arias in Resident Evil Vendetta to create the A-Virus. Later on, new Plaga variants would be engineered by an organization called Tricell, which is directly .